Nucleic Acids Research Advance Access published online on April 2, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm121
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Methods Online |
Parallel gene synthesis in a microfluidic device
1Center for Bits and Atoms, 2Media Laboratory, 3Department of Chemical Engineering and 4Center for Biomedical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
*To whom correspondence should be addressed. Tel: 617-253-7209; Fax: 617-253-6264; Email: jacobson{at}media.mit.edu
Received September 24, 2006. Revised January 27, 2007. Accepted February 13, 2007.
The ability to synthesize custom de novo DNA constructs rapidly, accurately and inexpensively is highly desired by researchers, as synthetic genes and longer DNA constructs are enabling to numerous powerful applications in both traditional molecular biology and the emerging field of synthetic biology. However, the current cost of de novo synthesis—driven largely by reagent and handling costs—is a significant barrier to the widespread availability of such technology. In this work, we demonstrate, to our knowledge, the first gene synthesis in a microfluidic environment. The use of microfluidic technology greatly reduces reaction volumes and the corresponding reagent and handling costs. Additionally, microfluidic technology enables large numbers of complex reactions to be performed in parallel. Here, we report the fabrication of a multi-chamber microfluidic device and its use in carrying out the syntheses of several DNA constructs. Genes up to 1 kb in length were synthesized in parallel at minute starting oligonucleotide concentrations (10–25 nM) in four 500 nl reactors. Such volumes are one to two orders of magnitude lower than those utilized in conventional gene synthesis. The identity of all target genes was verified by sequencing, and the resultant error rate was determined to be 1 per 560 bases.