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Nucleic Acids Research Advance Access published online on April 22, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm161
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© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Actin-related protein Arp4 functions in kinetochore assembly

Hideaki Ogiwara1, Ayako Ui1, Satoshi Kawashima1, Kazuto Kugou2,3, Fumitoshi Onoda1, Hitoshi Iwahashi4, Masahiko Harata5, Kunihiro Ohta2, Takemi Enomoto1,6 and Masayuki Seki1,*

1Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai, Miyagi 980-8578, Japan, 2Genetic System Regulation Laboratory, RIKEN, Wako, Saitama 351-0198, Japan, 3The Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama, Saitama 338-8570, Japan, 4National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan, 5Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 981-8555, Japan and 6Tohoku University 21st Century COE Program "Comprehensive Research and Education Center for Planning of Drug development and Clinical Evaluation", Sendai, Miyagi 980-8578, Japan

*To whom correspondence should be addressed. Tel: +81-22-795-6875; Fax: +81-22-795-6873; Email: seki{at}mail.pharm.tohoku.ac.jp

Received December 22, 2006. Revised February 27, 2007. Accepted March 2, 2007.

The actin-related proteins (Arps) comprise a conserved protein family. Arp4p is found in large multisubunits of the INO80 and SWR1 chromatin remodeling complexes and in the NuA4 histone acetyltransferase complex. Here we show that arp4 (arp4S23A/D159A) temperature-sensitive cells are defective in G2/M phase function. arp4 mutants are sensitive to the microtubule depolymerizing agent benomyl and arrest at G2/M phase at restrictive temperature. Arp4p is associated with centromeric and telomeric regions throughout cell cycle. Ino80p, Esa1p and Swr1p, components of the INO80, NuA4 and SWR1 complexes, respectively, also associate with centromeres. The association of many kinetochore components including Cse4p, a component of the centromere nucleosome, Mtw1p and Ctf3p is partially impaired in arp4 cells, suggesting that the G2/M arrest of arp4 mutant cells is due to a defect in formation of the chromosomal segregation apparatus.


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