Skip Navigation



Nucleic Acids Research Advance Access published online on March 28, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm175
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (4054K) Freely available
Right arrow Screen PDF (860K) Freely available
Right arrowOA All Versions of this Article:
35/7/2428    most recent
gkm175v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Mulder, K. W.
Right arrow Articles by Timmers, H. Th. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mulder, K. W.
Right arrow Articles by Timmers, H. Th. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Regulation of histone H3K4 tri-methylation and PAF complex recruitment by the Ccr4-Not complex

Klaas W. Mulder1, Arjan B. Brenkman2, Akiko Inagaki1, Niels J. F. van den Broek2 and H. Th. Marc Timmers1,*

1Department of Physiological Chemistry and 2Department of Physiological Chemistry Mass Spectrometry Unit, University Medical Centre Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands

*To whom correspondence should be addressed. Tel: +31-30-2538981; Fax: +31-30-2539035; Email: h.t.m.timmers{at}med.uu.nl

Received February 6, 2007. Revised March 8, 2007. Accepted March 8, 2007.

Efficient transcription is linked to modification of chromatin. For instance, tri-methylation of lysine 4 on histone H3 (H3K4) strongly correlates with transcriptional activity and is regulated by the Bur1/2 kinase complex. We found that the evolutionarily conserved Ccr4-Not complex is involved in establishing H3K4 tri-methylation in Saccharomyces cerevisiae. We observed synthetic lethal interactions of Ccr4-Not components with BUR1 and BUR2. Further analysis indicated that the genes encoding the Not-proteins are essential for efficient regulation of H3K4me3, but not H3K4me1/2, H3K36me2 or H3K79me2/3 levels. Moreover, regulation of H3K4me3 levels by NOT4 is independent of defects in RNA polymerase II loading. We found NOT4 to be important for ubiquitylation of histone H2B via recruitment of the PAF complex, but not for recruitment or activation of the Bur1/2 complex. These results suggest a mechanism in which the Ccr4-Not complex functions parallel to or downstream of the Bur1/2 kinase to facilitate H3K4me3 via PAF complex recruitment.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
A. Traven, T. H. Beilharz, T. L. Lo, F. Lueder, T. Preiss, and J. Heierhorst
The Ccr4-Pop2-NOT mRNA Deadenylase Contributes to Septin Organization in Saccharomyces cerevisiae
Genetics, August 1, 2009; 182(4): 955 - 966.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Zhou, W. H. W. Kuo, J. Fillingham, and J. F. Greenblatt
Control of transcriptional elongation and cotranscriptional histone modification by the yeast BUR kinase substrate Spt5
PNAS, April 28, 2009; 106(17): 6956 - 6961.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. N. Dimitrova, K. Kuroha, T. Tatematsu, and T. Inada
Nascent Peptide-dependent Translation Arrest Leads to Not4p-mediated Protein Degradation by the Proteasome
J. Biol. Chem., April 17, 2009; 284(16): 10343 - 10352.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
D. P. Mersman, H.-N. Du, I. M. Fingerman, P. F. South, and S. D. Briggs
Polyubiquitination of the demethylase Jhd2 controls histone methylation and gene expression
Genes & Dev., April 15, 2009; 23(8): 951 - 962.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.