Nucleic Acids Research Advance Access first published online on April 22, 2007
This version published online on May 25, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm234
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OligoCalc: an online oligonucleotide properties calculator
Robert H. Lurie Comprehensive Cancer Center, The Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
*To whom correspondence should be addressed. Tel: +1 312 695-1334; Fax: +1 847 510 0540; Email: wakibbe{at}northwestern.edu
Received January 30, 2007. Revised March 22, 2007. Accepted March 29, 2007.
We developed OligoCalc as a web-accessible, client-based computational engine for reporting DNA and RNA single-stranded and double-stranded properties, including molecular weight, solution concentration, melting temperature, estimated absorbance coefficients, inter-molecular self-complementarity estimation and intra-molecular hairpin loop formation. OligoCalc has a familiar ‘calculator’ look and feel, making it readily understandable and usable. OligoCalc incorporates three common methods for calculating oligonucleotide-melting temperatures, including a nearest-neighbor thermodynamic model for melting temperature. Since it first came online in 1997, there have been more than 900 000 accesses of OligoCalc from nearly 200 000 distinct hosts, excluding search engines. OligoCalc is available at http://basic.northwestern.edu/biotools/OligoCalc.html, with links to the full source code, usage patterns and statistics at that link as well.
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