Skip Navigation



Nucleic Acids Research Advance Access published online on May 5, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm243
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (8617K) Freely available
Right arrow Screen PDF (947K) Freely available
Right arrow Supplementary Material
Right arrowOA All Versions of this Article:
35/11/3551    most recent
gkm243v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Capp, J.-P.
Right arrow Articles by Canitrot, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Capp, J.-P.
Right arrow Articles by Canitrot, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Involvement of DNA polymerase µ in the repair of a specific subset of DNA double-strand breaks in mammalian cells

Jean-Pascal Capp1, François Boudsocq1, Anne-Gaelle Besnard1, Bernard S. Lopez2, Christophe Cazaux1, Jean-Sébastien Hoffmann1 and Yvan Canitrot1,*

1Genetic instability and Cancer group, Institute of Pharmacology and Structural Biology, University Paul Sabatier, UMR CNRS 5089, 205 route de Narbonne, 31077 Toulouse cedex and 2Département de Radiobiologie et Radiopathologie, 60-68 av. Général Leclerc, UMR 217 CNRS-CEA, 92265 Fontenay aux Roses cedex, France

*To whom correspondence should be addressed. Tel: +33 5 6117 5861; Fax: +33 5 6117 5994; Email: Yvan.Canitrot{at}ipbs.fr Correspondence may also be addressed to Jean-Sebastien Hoffmann. Tel: +33 5 6117 5975; Fax: +33 5 6117 5994; Email: jseb{at}ipbs.fr

Received February 27, 2007. Revised April 2, 2007. Accepted April 3, 2007.

The repair of DNA double-strand breaks (DSB) requires processing of the broken ends to complete the ligation process. Recently, it has been shown that DNA polymerase µ (polµ) and DNA polymerase {lambda} (pol{lambda}) are both involved in such processing during non-homologous end joining in vitro. However, no phenotype was observed in animal models defective for either polµ and/or pol{lambda}. Such observations could result from a functional redundancy shared by the X family of DNA polymerases. To avoid such redundancy and to clarify the role of polµ in the end joining process, we generated cells over-expressing the wild type as well as an inactive form of polµ (polµD). We observed that cell sensitivity to ionizing radiation (IR) was increased when either polµ or polµD was over-expressed. However, the genetic instability in response to IR increased only in cells expressing polµD. Moreover, analysis of intrachromosomal repair of the I-SceI-induced DNA DSB, did not reveal any effect of either polµ or polµD expression on the efficiency of ligation of both cohesive and partially complementary ends. Finally, the sequences of the repaired ends were specifically affected when polµ or polµD was over-expressed, supporting the hypothesis that polµ could be involved in the repair of a DSB subset when resolution of junctions requires some gap filling.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. Covo, J.-P. de Villartay, P. A. Jeggo, and Z. Livneh
Translesion DNA synthesis-assisted non-homologous end-joining of complex double-strand breaks prevents loss of DNA sequences in mammalian cells
Nucleic Acids Res., November 1, 2009; 37(20): 6737 - 6745.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Romain, I. Barbosa, J. Gouge, F. Rougeon, and M. Delarue
Conferring a template-dependent polymerase activity to terminal deoxynucleotidyltransferase by mutations in the Loop1 region
Nucleic Acids Res., August 1, 2009; 37(14): 4642 - 4656.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. B. Gammon and D. H. Evans
The 3'-to-5' Exonuclease Activity of Vaccinia Virus DNA Polymerase Is Essential and Plays a Role in Promoting Virus Genetic Recombination
J. Virol., May 1, 2009; 83(9): 4236 - 4250.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Nakane, N. Nakagawa, S. Kuramitsu, and R. Masui
Characterization of DNA polymerase X from Thermus thermophilus HB8 reveals the POLXc and PHP domains are both required for 3'-5' exonuclease activity
Nucleic Acids Res., April 1, 2009; 37(6): 2037 - 2052.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. Gozalbo-Lopez, P. Andrade, G. Terrados, B. de Andres, N. Serrano, I. Cortegano, B. Palacios, A. Bernad, L. Blanco, M. A. R. Marcos, et al.
A Role for DNA Polymerase {micro} in the Emerging DJH Rearrangements of the Postgastrulation Mouse Embryo
Mol. Cell. Biol., March 1, 2009; 29(5): 1266 - 1275.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. Leduc, V. Maquennehan, G. B. Nkoma, and G. Boissonneault
DNA Damage Response During Chromatin Remodeling in Elongating Spermatids of Mice
Biol Reprod, February 1, 2008; 78(2): 324 - 332.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. E. Haber
Alternative endings
PNAS, January 15, 2008; 105(2): 405 - 406.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Guirouilh-Barbat, E. Rass, I. Plo, P. Bertrand, and B. S. Lopez
Defects in XRCC4 and KU80 differentially affect the joining of distal nonhomologous ends
PNAS, December 26, 2007; 104(52): 20902 - 20907.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.