Nucleic Acids Research Advance Access published online on May 21, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm259
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WoLF PSORT: protein localization predictor
1Computational Biology Research Center, AIST, Tokyo, Japan, 2Center for Genome Science, National Institute of Health, Korea Center for Disease Control & Prevention, 5 Nokbeon-Dong, Eunpyung-Gu, Seoul 122-701 Korea, 3Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan and 4Collier Technologies, Everett, WA, USA
*To whom correspondence should be addressed. Tel: + 81-3-5449-5131; Fax: + 81-3-5449-5133; Email: knakai{at}ims.u-tokyo.ac.jp
Received January 30, 2007. Revised March 26, 2007. Accepted April 8, 2007.
WoLF PSORT is an extension of the PSORT II program for protein subcellular location prediction. WoLF PSORT converts protein amino acid sequences into numerical localization features; based on sorting signals, amino acid composition and functional motifs such as DNA-binding motifs. After conversion, a simple k-nearest neighbor classifier is used for prediction. Using html, the evidence for each prediction is shown in two ways: (i) a list of proteins of known localization with the most similar localization features to the query, and (ii) tables with detailed information about individual localization features. For convenience, sequence alignments of the query to similar proteins and links to UniProt and Gene Ontology are provided. Taken together, this information allows a user to understand the evidence (or lack thereof) behind the predictions made for particular proteins. WoLF PSORT is available at wolfpsort.org
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