Nucleic Acids Research Advance Access published online on May 21, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm314
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
RNA |
Regulation of glutamate receptor B pre-mRNA splicing by RNA editing
Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Dr Bohr-Gasse 1, A-1030 Vienna, Austria
*To whom correspondence should be addressed. Tel: +43 1 4277 56230; Fax: +43 1 4277 9562; Email: michael.jantsch{at}univie.ac.at
Received February 12, 2007. Revised April 11, 2007. Accepted April 13, 2007.
RNA-editing enzymes of the ADAR family convert adenosines to inosines in double-stranded RNA substrates. Frequently, editing sites are defined by base-pairing of the editing site with a complementary intronic region. The glutamate receptor subunit B (GluR-B) pre-mRNA harbors two such exonic editing sites termed Q/R and R/G. Data from ADAR knockout mice and in vitro editing assays suggest an intimate connection between editing and splicing of GluR-B pre-mRNA.
By comparing the events at the Q/R and R/G sites, we can show that editing can both stimulate and repress splicing efficiency. The edited nucleotide, but not ADAR binding itself, is sufficient to exert this effect. The presence of an edited nucleotide at the R/G site reduces splicing efficiency of the adjacent intron facilitating alternative splicing events occurring downstream of the R/G site.
Lack of editing inhibits splicing at the Q/R site. Editing of both the Q/R nucleotide and an intronic editing hotspot are required to allow efficient splicing. Inefficient intron removal may ensure that only properly edited mRNAs become spliced and exported to the cytoplasm.
Present address: Vera K. Schoft, Gregor Mendel Institute, Dr Bohr-Gasse 3, A-1030 Vienna, Austria
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. K. Jones, S. D. Buckingham, M. Papadaki, M. Yokota, B. M. Sattelle, K. Matsuda, and D. B. Sattelle Splice-Variant- and Stage-Specific RNA Editing of the Drosophila GABA Receptor Modulates Agonist Potency J. Neurosci., April 1, 2009; 29(13): 4287 - 4292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Flanagan, J. M. Funes, S. Henderson, L. Wild, N. Carey, and C. Boshoff Genomics screen in transformed stem cells reveals RNASEH2A, PPAP2C, and ADARB1 as putative anticancer drug targets Mol. Cancer Ther., January 1, 2009; 8(1): 249 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yang, J. Lv, B. Gui, H. Yin, X. Wu, Y. Zhang, and Y. Jin A-to-I RNA editing alters less-conserved residues of highly conserved coding regions: Implications for dual functions in evolution RNA, August 1, 2008; 14(8): 1516 - 1525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Tian, X. Wu, Y. Zhang, and Y. Jin A-to-I editing sites are a genomically encoded G: Implications for the evolutionary significance and identification of novel editing sites RNA, February 1, 2008; 14(2): 211 - 216. [Abstract] [Full Text] [PDF]
|
|