Skip Navigation



Nucleic Acids Research Advance Access published online on May 30, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm383
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (581K) Freely available
Right arrow Screen PDF (132K) Freely available
Right arrowOA All Versions of this Article:
35/suppl_2/W63    most recent
gkm383v3
gkm383v2
gkm383v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Tsai, M.-F.
Right arrow Articles by Yao, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsai, M.-F.
Right arrow Articles by Yao, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Web Server Issue

PrimerZ: streamlined primer design for promoters, exons and human SNPs

Ming-Fang Tsai1, Yi-Jung Lin1, Yu-Chang Cheng1, Kuo-Hsi Lee1, Cheng-Chih Huang2, Yuan-Tsong Chen1,2 and Adam Yao1,2,*

1National Genotyping Center (NGC), Academia Sinica, Taipei, Taiwan 11529, R.O.C. and 2Institute of Biomedical Sciences (IBMS), Academia Sinica, Taipei, Taiwan 11529, R.O.C.

*To whom correspondence should be addressed. Tel: +886 2 26523091; Fax: +886 2 27824066; Email: adam{at}ibms.sinica.edu.tw

Received January 31, 2007. Revised April 27, 2007. Accepted April 30, 2007.

PrimerZ (http://genepipe.ngc.sinica.edu.tw/primerz/) is a web application dedicated primarily to primer design for genes and human SNPs. PrimerZ accepts genes by gene name or Ensembl accession code, and SNPs by dbSNP rs or AFFY_Probe IDs. The promoter and exon sequence information of all gene transcripts fetched from the Ensembl database (http://www.ensembl.org/) are processed before being passed on to Primer3 (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi) for individual primer design. All results returned from Primer 3 are organized and integrated in a specially designed web page for easy browsing. Besides the web page presentation, csv text file export is also provided for enhanced user convenience.

PrimerZ automates highly standard but tedious gene primer design to improve the success rate of PCR experiments. More than 2000 primers have been designed with PrimerZ at our institute since 2004 and the success rate is over 70%. The addition of several new features has made PrimerZ even more useful to the research community in facilitating primer design for promoters, exons and SNPs.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
Y.-H. Chen, C.-K. Liu, S.-C. Chang, Y.-J. Lin, M.-F. Tsai, Y.-T. Chen, and A. Yao
GenoWatch: a disease gene mining browser for association study
Nucleic Acids Res., April 25, 2008; (2008) gkn214v1.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.