Skip Navigation



Nucleic Acids Research Advance Access published online on August 1, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm566
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1124K) Freely available
Right arrow Screen PDF (281K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
35/15/e97    most recent
gkm566v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Meyer, M.
Right arrow Articles by Hofreiter, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meyer, M.
Right arrow Articles by Hofreiter, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Methods Online

Targeted high-throughput sequencing of tagged nucleic acid samples

Matthias Meyer*, Udo Stenzel, Sean Myles, Kay Prüfer and Michael Hofreiter

Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, D-04103 Leipzig, Germany

*To whom correspondence should be addressed. Tel: +49 341 3550 509, Fax: +49 341 3550 555; Email: mmeyer{at}eva.mpg.de

Received May 30, 2007. Revised June 21, 2007. Accepted July 6, 2007.

High-throughput 454 DNA sequencing tech-nology allows much faster and more cost-effective sequencing than traditional Sanger sequencing. However, the technology imposes inherent limitations on the number of samples that can be processed in parallel. Here we introduce parallel tagged sequencing (PTS), a simple, inexpensive and flexible barcoding technique that can be used for parallel sequencing any number and type of double-stranded nucleic acid samples. We demonstrate that PTS is particularly powerful for sequencing contiguous DNA fragments such as mtDNA genomes: in theory as many as 250 mammalian mtDNA genomes can be sequenced in a single GS FLX run. PTS dramatically increases the sequencing throughput of samples in parallel and thus fully mobilizes the resources of the 454 technology for targeted sequencing.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 The Plant GenomeHome page
C. Zhu, M. Gore, E. S. Buckler, and J. Yu
Status and Prospects of Association Mapping in Plants
The Plant Genome, July 1, 2008; 1(1): 5 - 20.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Meyer, A. W. Briggs, T. Maricic, B. Hober, B. Hoffner, J. Krause, A. Weihmann, S. Paabo, and M. Hofreiter
From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing
Nucleic Acids Res., January 17, 2008; 36(1): e5 - e5.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.