Skip Navigation



Nucleic Acids Research Advance Access published online on August 13, 2007
Nucleic Acids Research, doi:10.1093/nar/gkm599
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (2218K) Freely available
Right arrow Screen PDF (260K) Freely available
Right arrow Supplementary Material
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
35/16/5402    most recent
gkm599v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Agarwal, N.
Right arrow Articles by Cardoso, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Agarwal, N.
Right arrow Articles by Cardoso, M. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation

Noopur Agarwal1, Tanja Hardt1, Alessandro Brero1, Danny Nowak1, Ulrich Rothbauer2, Annette Becker1, Heinrich Leonhardt2 and M. Cristina Cardoso1,*

1Max Delbrück Center for Molecular Medicine, 13125 Berlin and 2Ludwig Maximilians University Munich, Biocenter, Department of Biology, 82152 Planegg-Martinsried, Germany

*To whom Correspondence should be addressed: Tel: +49 30 94062109; Fax: +49 30 94063343; Email: cardoso{at}mdc-berlin.de

Received March 7, 2007. Revised July 20, 2007. Accepted July 20, 2007.

There is increasing evidence of crosstalk between epigenetic modifications such as histone and DNA methylation, recognized by HP1 and methyl CpG-binding proteins, respectively. We have previously shown that the level of methyl CpG-binding proteins increased dramatically during myogenesis leading to large-scale heterochromatin reorganization. In this work, we show that the level of HP1 isoforms did not change significantly throughout myogenic differentiation but their localization did. In particular, HP1{gamma} relocalization to heterochromatin correlated with MeCP2 presence. Using co-immunoprecipitation assays, we found that these heterochromatic factors interact in vivo via the chromo shadow domain of HP1 and the first 55 amino acids of MeCP2. We propose that this dynamic interaction of HP1 and MeCP2 increases their concentration at heterochromatin linking two major gene silencing pathways to stabilize transcriptional repression during differentiation.

Present address: Tanja Hardt, Medical Proteomics Center, Ruhr University, 44801, Germany

Alessandro Brero, Ludwig Maximilians University, Gene Center, 81377 Munich, Germany


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
H. Yahi, L. Fritsch, O. Philipot, V. Guasconi, M. Souidi, P. Robin, A. Polesskaya, R. Losson, A. Harel-Bellan, and S. Ait-Si-Ali
Differential Cooperation between Heterochromatin Protein HP1 Isoforms and MyoD in Myoblasts
J. Biol. Chem., August 29, 2008; 283(35): 23692 - 23700.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Kumar, S. Kamboj, B. M. Malone, S. Kudo, J. L. Twiss, K. J. Czymmek, J. M. LaSalle, and N. C. Schanen
Analysis of protein domains and Rett syndrome mutations indicate that multiple regions influence chromatin-binding dynamics of the chromatin-associated protein MECP2 in vivo
J. Cell Sci., April 1, 2008; 121(7): 1128 - 1137.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.