Skip Navigation



Nucleic Acids Research Advance Access published online on February 5, 2008
Nucleic Acids Research, doi:10.1093/nar/gkn005
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1157K) Freely available
Right arrow Screen PDF (691K) Freely available
Right arrowOA All Versions of this Article:
36/6/1792    most recent
gkn005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Mirkin, N.
Right arrow Articles by Kleiman, F. E.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mirkin, N.
Right arrow Articles by Kleiman, F. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

RNA

The 3' processing factor CstF functions in the DNA repair response

Nurit Mirkin1, Danae Fonseca1, Samia Mohammed1, Murat A. Cevher1, James L. Manley2 and Frida E. Kleiman1,*

1Chemistry Department, Hunter College, City University of New York and 2Department of Biological Sciences, Columbia University, New York, NY 10027, USA

*To whom correspondence should be addressed. Tel: +1 212 772 5355; Fax: +1 212 772 5332; Email: fkleiman{at}hunter.cuny.edu

Received September 10, 2007. Revised January 7, 2008. Accepted January 7, 2008.

Following DNA damage, mRNA levels decrease, reflecting a coordinated interaction of the DNA repair, transcription and RNA processing machineries. In this study, we provide evidence that transcription and polyadenylation of mRNA precursors are both affected in vivo by UV treatment. We next show that the polyadenylation factor CstF, plays a direct role in the DNA damage response. Cells with reduced levels of CstF display decreased viability following UV treatment, reduced ability to ubiquitinate RNA polymerase II (RNAP II), and defects in repair of DNA damage. Furthermore, we show that CstF, RNAP II and BARD1 are all found at sites of repaired DNA. Our results indicate that CstF plays an active role in the response to DNA damage, providing a link between transcription-coupled RNA processing and DNA repair.

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.