Skip Navigation



Nucleic Acids Research Advance Access published online on March 19, 2008
Nucleic Acids Research, doi:10.1093/nar/gkn032
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (957K) Freely available
Right arrow Screen PDF (496K) Freely available
Right arrowOA All Versions of this Article:
36/8/2690    most recent
gkn032v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Sun, Q.
Right arrow Articles by Yang, X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sun, Q.
Right arrow Articles by Yang, X.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Transforming growth factor-β-regulated miR-24 promotes skeletal muscle differentiation

Qiang Sun1, Yan Zhang1, Guang Yang2, Xiaoping Chen3, Yingai Zhang1, Guojun Cao2, Jian Wang1, Yanxun Sun1, Peng Zhang3, Ming Fan2, Ningsheng Shao2 and Xiao Yang1,*

1State Key Laboratory of Proteomics, Genetic Laboratory of Development and Diseases, Institute of Biotechnology, 20 Dongdajie, Beijing 100071, 2Institute of Basic Medical Sciences, 27 Taiping Road, Beijing 100850 and 3China Astronaut Research and Training Center, Yuanmingyuan West Road, Beijing 100094, P. R. China

*To whom correspondence should be addressed. Tel: +86 10 63895937; Fax: +86 10 63895937; Email: yangx{at}nic.bmi.ac.cn Correspondence may also be addressed to Ningsheng Shao. Tel: +86 10 68163140; Fax: +86 10 68163140; Email: shaons{at}yahoo.com

Received December 29, 2007. Accepted January 19, 2008.

MicroRNAs (miRNAs) have recently been proposed as a versatile class of molecules involved in regulation of a variety of biological processes. However, the role of miRNAs in TGF-β-regulated biological processes is poorly addressed. In this study, we found that miR-24 was upregulated during myoblast differentiation and could be inhibited by TGF-β1. Using both a reporter assay and Northern blot analysis, we showed that TGF-β1 repressed miR-24 transcription which was dependent on the presence of Smad3 and a Smads binding site in the promoter region of miR-24. TGF-β1 was unable to inhibit miR-24 expression in Smad3-deficient myoblasts, which exhibited accelerated myogenesis. Knockdown of miR-24 led to reduced expression of myogenic differentiation markers in C2C12 cells, while ectopic expression of miR-24 enhanced differentiation, and partially rescued inhibited myogenesis by TGF-β1. This is the first study demonstrating a critical role for miRNAs in modulating TGF-β-dependent inhibition of myogenesis, and provides a novel mechanism of the genetic regulation of TGF-β signaling during skeletal muscle differentiation.

The authors wish it to be known that, in their opinion, the first three authors should be regarded as joint First Authors.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
A. Moustakas and C.-H. Heldin
The regulation of TGF{beta} signal transduction
Development, November 15, 2009; 136(22): 3699 - 3714.
[Abstract] [Full Text] [PDF]

Home page
 CJASNHome page
M. Kato, L. Arce, and R. Natarajan
MicroRNAs and Their Role in Progressive Kidney Diseases
Clin. J. Am. Soc. Nephrol., July 1, 2009; 4(7): 1255 - 1266.
[Abstract] [Full Text] [PDF]

Home page
 Acta Biochim Biophys SinHome page
B. Huang, W. Qin, B. Zhao, Y. Shi, C. Yao, J. Li, H. Xiao, and Y. Jin
MicroRNA expression profiling in diabetic GK rat model
Acta Biochim Biophys Sin, June 1, 2009; 41(6): 472 - 477.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. Brock, M. Trenkmann, R. E. Gay, B. A. Michel, S. Gay, M. Fischler, S. Ulrich, R. Speich, and L. C. Huber
Interleukin-6 Modulates the Expression of the Bone Morphogenic Protein Receptor Type II Through a Novel STAT3-microRNA Cluster 17/92 Pathway
Circ. Res., May 22, 2009; 104(10): 1184 - 1191.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Yang, L. Wang, and X. Yang
Disruption of Smad4 in Mouse Epidermis Leads to Depletion of Follicle Stem Cells
Mol. Biol. Cell, February 1, 2009; 20(3): 882 - 890.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
G. Yang, Q. Sun, Y. Teng, F. Li, T. Weng, and X. Yang
PTEN deficiency causes dyschondroplasia in mice by enhanced hypoxia-inducible factor 1{alpha} signaling and endoplasmic reticulum stress
Development, November 1, 2008; 135(21): 3587 - 3597.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.