Skip Navigation



Nucleic Acids Research Advance Access published online on February 14, 2008
Nucleic Acids Research, doi:10.1093/nar/gkn046
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (2071K) Freely available
Right arrow Screen PDF (1360K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
36/6/2032    most recent
gkn046v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Rodionov, D. A.
Right arrow Articles by Osterman, A. L.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rodionov, D. A.
Right arrow Articles by Osterman, A. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Genomics

Transcriptional regulation of NAD metabolism in bacteria: genomic reconstruction of NiaR (YrxA) regulon

Dmitry A. Rodionov1,2,*, Xiaoqing Li1, Irina A. Rodionova1, Chen Yang1, Leonardo Sorci1, Etienne Dervyn3, Dariusz Martynowski4, Hong Zhang4, Mikhail S. Gelfand2 and Andrei L. Osterman1,5

1Burnham Institute for Medical Research, La Jolla, CA 92037, USA, 2Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow 127994, Russia, 3Génétique Microbienne, INRA, 78352 Jouy-en-Josas, France, 4Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390 and 5Fellowship for Interpretation of Genomes, Burr Ridge, IL 60527, USA

*To whom correspondence should be addressed. Tel: +1 858 646 3100; Fax: +1 858 795 5249_; Email: rodionov{at}burnham.org

Received December 5, 2007. Revised January 18, 2008. Accepted January 24, 2008.

A comparative genomic approach was used to reconstruct transcriptional regulation of NAD biosynthesis in bacteria containing orthologs of Bacillus subtilis gene yrxA, a previously identified niacin-responsive repressor of NAD de novo synthesis. Members of YrxA family (re-named here NiaR) are broadly conserved in the Bacillus/Clostridium group and in the deeply branching Fusobacteria and Thermotogales lineages. We analyzed upstream regions of genes associated with NAD biosynthesis to identify candidate NiaR-binding DNA motifs and assess the NiaR regulon content in these species. Representatives of the two distinct types of candidate NiaR-binding sites, characteristic of the Firmicutes and Thermotogales, were verified by an electrophoretic mobility shift assay. In addition to transcriptional control of the nadABC genes, the NiaR regulon in some species extends to niacin salvage (the pncAB genes) and includes uncharacterized membrane proteins possibly involved in niacin transport. The involvement in niacin uptake proposed for one of these proteins (re-named NiaP), encoded by the B. subtilis gene yceI, was experimentally verified. In addition to bacteria, members of the NiaP family are conserved in multicellular eukaryotes, including human, pointing to possible NaiP involvement in niacin utilization in these organisms. Overall, the analysis of the NiaR and NrtR regulons (described in the accompanying paper) revealed mechanisms of transcriptional regulation of NAD metabolism in nearly a hundred diverse bacteria.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.