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Nucleic Acids Research Advance Access published online on July 16, 2008
Nucleic Acids Research, doi:10.1093/nar/gkn424
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© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

RNA

Features of 80S mammalian ribosome and its subunits

Tatyana V. Budkevich1,2, Anna V. El'skaya2 and Knud H. Nierhaus1,*

1Max-Planck-Institut für Molekulare Genetik, Ihnestr. 73, D-14195 Berlin, Germany and 2Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150, Zabolotnogo str., Kiev, 03143, Ukraine

*To whom correspondence should be addressed. Tel: +49 30 8413 1700; Fax: +49 30 8413 1594; Email: nierhaus{at}molgen.mpg.de

Received May 9, 2008. Revised June 18, 2008. Accepted June 19, 2008.

It is generally believed that basic features of ribosomal functions are universally valid, but a systematic test still stands out for higher eukaryotic 80S ribosomes. Here we report: (i) differences in tRNA and mRNA binding capabilities of eukaryotic and bacterial ribosomes and their subunits. Eukaryotic 40S subunits bind mRNA exclusively in the presence of cognate tRNA, whereas bacterial 30S do bind mRNA already in the absence of tRNA. 80S ribosomes bind mRNA efficiently in the absence of tRNA. In contrast, bacterial 70S interact with mRNA more productively in the presence rather than in the absence of tRNA. (ii) States of initiation (Pi), pre-translocation (PRE) and post-translocation (POST) of the ribosome were checked and no significant functional differences to the prokaryotic counterpart were observed including the reciprocal linkage between A and E sites. (iii) Eukaryotic ribosomes bind tetracycline with an affinity 15 times lower than that of bacterial ribosomes (Kd 30 µM and 1–2 µM, respectively). The drug does not effect enzymatic A-site occupation of 80S ribosomes in contrast to non-enzymatic tRNA binding to the A-site. Both observations explain the relative resistance of eukaryotic ribosomes to this antibiotic.

Present address: Tatyana V. Budkevich, Institut für Medizinische Physik und Biophysik, Charité – Universitätsmedizin Berlin, Ziegelstr. 5-9, 10117 Berlin, Germany


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