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Nucleic Acids Research Advance Access published online on August 5, 2008
Nucleic Acids Research, doi:10.1093/nar/gkn465
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© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Positive roles of SAS2 in DNA replication and transcriptional silencing in yeast

Yanfei Zou and Xin Bi*

Department of Biology, University of Rochester, Rochester, NY 14627, USA

*To whom correspondence should be addressed. Tel: +1 585 275 6922; Fax: +1 585 275 2070; Email: xinbi{at}mail.rochester.edu

Received May 11, 2008. Revised June 25, 2008. Accepted July 3, 2008.

Sas2p is a histone acetyltransferase implicated in the regulation of transcriptional silencing, and ORC is the six-subunit origin recognition complex involved in the initiation of DNA replication and the establishment of transcriptionally silent chromatin by silencers in yeast. We show here that SAS2 deletion (sas2{Delta}) exacerbates the temperature sensitivity of the ORC mutants orc2-1 and orc5-1. Moreover, sas2{Delta} and orc2-1 have a synthetic effect on cell cycle progression through S phase and initiation of DNA replication. These results suggest that SAS2 plays a positive role in DNA replication and cell cycle progression. We also show that sas2{Delta} and orc5-1 have a synthetic effect on transcriptional silencing at the HMR locus. Moreover, we demonstrate that sas2{Delta} reduces the silencing activities of silencers regardless of their locations and contexts, indicating that SAS2 plays a positive role in silencer function. In addition, we show that SAS2 is required for maintaining the structure of transcriptionally silent chromatin.


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