Skip Navigation



Nucleic Acids Research Advance Access published online on October 3, 2008
Nucleic Acids Research, doi:10.1093/nar/gkn624
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (4494K) Freely available
Right arrow Screen PDF (758K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
36/19/6318    most recent
gkn624v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ghosh, T.
Right arrow Articles by Pillai, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ghosh, T.
Right arrow Articles by Pillai, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

RNA

MicroRNA-mediated up-regulation of an alternatively polyadenylated variant of the mouse cytoplasmic β-actin gene

Tanay Ghosh, Kartik Soni, Vinod Scaria, Mahantappa Halimani, Chaitali Bhattacharjee and Beena Pillai*

Institute of Genomics and Integrative Biology (IGIB), Mall Road, New Delhi 110007, India

*To whom correspondence should be addressed. Tel: +91 011 27666156; Fax: +91 011 27667471; Email: beenapillai{at}igib.res.in

Received February 2, 2008. Revised September 8, 2008. Accepted September 12, 2008.

Actin is a major cytoskeletal protein in eukaryotes. Recent studies suggest more diverse functional roles for this protein. Actin mRNA is known to be localized to neuronal synapses and undergoes rapid deadenylation during early developmental stages. However, its 3'-untranslated region (UTR) is not characterized and there are no experimentally determined polyadenylation (polyA) sites in actin mRNA. We have found that the cytoplasmic β-actin (Actb) gene generates two alternative transcripts terminated at tandem polyA sites. We used 3'-RACE, EST end analysis and in situ hybridization to unambiguously establish the existence of two 3'-UTRs of varying length in Actb transcript in mouse neuronal cells. Further analyses showed that these two tandem polyA sites are used in a tissue-specific manner. Although the longer 3'-UTR was expressed at a relatively lower level, it conferred higher translational efficiency to the transcript. The longer transcript harbours a conserved mmu-miR-34a/34b-5p target site. Sequence-specific anti-miRNA molecule, mutations of the miRNA target region in the 3'-UTR resulted in reduced expression. The expression was restored by a mutant miRNA complementary to the mutated target region implying that miR-34 binding to Actb 3'-UTR up-regulates target gene expression. Heterogeneity of the Actb 3'-UTR could shed light on the mechanism of miRNA-mediated regulation of messages in neuronal cells.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genome ResHome page
A. S. Morrissy, R. D. Morin, A. Delaney, T. Zeng, H. McDonald, S. Jones, Y. Zhao, M. Hirst, and M. A. Marra
Next-generation tag sequencing for cancer gene expression profiling
Genome Res., October 1, 2009; 19(10): 1825 - 1835.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
F. Song, H. Zheng, B. Liu, S. Wei, H. Dai, L. Zhang, G. A. Calin, X. Hao, Q. Wei, W. Zhang, et al.
An miR-502-Binding Site Single-Nucleotide Polymorphism in the 3'-Untranslated Region of the SET8 Gene Is Associated with Early Age of Breast Cancer Onset
Clin. Cancer Res., October 1, 2009; 15(19): 6292 - 6300.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.