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Nucleic Acids Research Advance Access published online on October 16, 2008
Nucleic Acids Research, doi:10.1093/nar/gkn703
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© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

RNA

Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNAMet

Hrvoje Lusic1, Estella M. Gustilo2, Franck A.P. Vendeix2, Rob Kaiser3, Michael O. Delaney3, William D. Graham2, Virginia A. Moye2, William A. Cantara2, Paul F. Agris2,* and Alexander Deiters1

1Department of Chemistry, 2Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695 and 3Dharmacon, 2650 Crescent Drive #100, Lafayette, CO 80026, USA

*To whom correspondence should be addressed. Tel: +1 919 515 6188; Fax: +1 919 515 2047; Email: Paul_Agris{at}ncsu.edu

Correspondence may also be addressed to Alexander Deiters. Tel: +1 919 513 2958; Fax: +1 919 515 5079; Email: Alex_Deiters{at}ncsu.edu

Received July 29, 2008. Revised September 28, 2008. Accepted September 29, 2008.

Human mitochondrial methionine transfer RNA (hmtRNAFormula) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f5C34). The role of this modification in (hmtRNAFormula) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification. The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation.

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.


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C. Takemoto, L. L. Spremulli, L. A. Benkowski, T. Ueda, T. Yokogawa, and K. Watanabe
Unconventional decoding of the AUA codon as methionine by mitochondrial tRNAMet with the anticodon f5CAU as revealed with a mitochondrial in vitro translation system
Nucleic Acids Res., April 1, 2009; 37(5): 1616 - 1627.
[Abstract] [Full Text] [PDF]


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