Skip Navigation



Nucleic Acids Research Advance Access published online on October 31, 2008
Nucleic Acids Research, doi:10.1093/nar/gkn818
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (3337K) Freely available
Right arrow Screen PDF (462K) Freely available
Right arrowOA All Versions of this Article:
36/21/6926    most recent
gkn818v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Pérez-Lluch, S.
Right arrow Articles by Espinàs, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pérez-Lluch, S.
Right arrow Articles by Espinàs, M. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Gene regulation, Chromatin and Epigenetics

Characterization of new regulatory elements within the Drosophila bithorax complex

Sílvia Pérez-Lluch1,2, Sergi Cuartero1,2, Fernando Azorín1,2 and M. Lluïsa Espinàs1,2,*

1Institut de Biologia Molecular de Barcelona, CSIC and 2Institut de Recerca Biomedica de Barcelona, IRBB, Parc Científic de Barcelona, Baldiri Reixac 10-12, 08028 Barcelona, Spain

*To whom correspondence should be addressed. Tel: +34 934034962; Fax: +34 934034979; Email: mlebmc{at}ibmb.csic.es Present address: Sílvia Pérez-Lluch, Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain

Received September 9, 2008. Revised October 10, 2008. Accepted October 13, 2008.

The homeotic Abdominal-B (Abd-B) gene expression depends on a modular cis-regulatory region divided into discrete functional domains (iab) that control the expression of the gene in a particular segment of the fly. These domains contain regulatory elements implicated in both initiation and maintenance of homeotic gene expression and elements that separate the different domains. In this paper we have performed an extensive analysis of the iab-6 regulatory region, which regulates Abd-B expression at abdominal segment A6 (PS11), and we have characterized two new polycomb response elements (PREs) within this domain. We report that PREs at Abd-B cis-regulatory domains present a particular chromatin structure which is nuclease accessible all along Drosophila development and both in active and repressed states. We also show that one of these regions contains a dCTCF and CP190 dependent activity in transgenic enhancer-blocking assays, suggesting that it corresponds to the Fab-6 boundary element of the Drosophila bithorax complex.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.