Skip Navigation



Nucleic Acids Research Advance Access published online on February 25, 2009
Nucleic Acids Research, doi:10.1093/nar/gkp090
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (5323K) Freely available
Right arrow Screen PDF (698K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
37/7/2313    most recent
gkp090v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Dickson, A. M.
Right arrow Articles by Wold, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dickson, A. M.
Right arrow Articles by Wold, M. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2009 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Genome Integrity, Repair and Replication

Essential functions of the 32 kDa subunit of yeast replication protein A

Anne M. Dickson1, Yulia Krasikova2, Pavel Pestryakov2, Olga Lavrik2 and Marc S. Wold1,*

1Department of Biochemistry, University of Iowa College of Medicine, Iowa City, IA 52242-2600, USA and 2Novosibirsk Institute of Bioorganic Chemistry, Prospekt Lavrentiev 8, 630090 Novosibirsk, Russia

*To whom correspondence should be addressed. Tel: +1 319 335 6784; Fax: +1 319 384 4770; Email: marc-wold{at}uiowa.edu

Received December 1, 2008. Revised January 30, 2009. Accepted February 4, 2009.

Replication protein A (RPA) is a heterotrimeric (70, 32 and 14 kDa subunits), single-stranded DNA-binding protein required for cellular DNA metabolism. All subunits of RPA are essential for life, but the specific functions of the 32 and 14 kDa subunits remains unknown. The 32 kDa subunit (RPA2) has multiple domains, but only the central DNA-binding domain (called DBD D) is essential for life in Saccharomyces cerevisiae. To define the essential function(s) of RPA2 in S. cerevisiae, a series of site-directed mutant forms of DBD D were generated. These mutant constructs were then characterized in vitro and in vivo. The mutations had minimal effects on the overall structure and activity of the RPA complex. However, several mutants were shown to disrupt crosslinking of RPA2 to DNA and to dramatically lower the DNA-binding affinity of a RPA2-containing subcomplex. When introduced into S. cerevisiae, all DBD D mutants were viable and supported normal growth rates and DNA replication. These findings indicate that RPA2–DNA interactions are not essential for viability and growth in S. cerevisiae. We conclude that DNA-binding activity of RPA2 is dispensable in yeast and that the essential function of DBD D is intra- and/or inter-protein interactions.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. J. Haring, T. D. Humphreys, and M. S. Wold
A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression
Nucleic Acids Res., November 26, 2009; (2009) gkp1062v1.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.