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Nucleic Acids Research Advance Access published online on March 31, 2009
Nucleic Acids Research, doi:10.1093/nar/gkp198
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© 2009
The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Structural Biology

Structural basis for the specialization of Nur, a nickel-specific Fur homolog, in metal sensing and DNA recognition

Young Jun An1,2, Bo-Eun Ahn3, A-Reum Han3, Hae-Mi Kim3, Kyung Min Chung4, Jung-Ho Shin3, Yoo-Bok Cho3, Jung-Hye Roe3,* and Sun-Shin Cha1,*

1Marine and Extreme Genome Research Center, Korea Ocean Research & Development Institute, Ansan 426-744, 2Department of Biological Sciences, Myongji University, Yongin 449-728, 3School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151-742 and 4Department of Microbiology, Chonbuk National University Medical School, Chonju, Chonbuk, 561-180, Republic of Korea

*To whom correspondence should be addressed. Tel: +82 31 400 6297; Fax: +82 31 406 2495; Email: chajung{at}kordi.re.kr

Received October 21, 2008. Revised March 11, 2009. Accepted March 11, 2009.

Nur, a member of the Fur family, is a nickel-responsive transcription factor that controls nickel homeostasis and anti-oxidative response in Streptomyces coelicolor. Here we report the 2.4-Å resolution crystal structure of Nur. It contains a unique nickel-specific metal site in addition to a nonspecific common metal site. The identification of the 6-5-6 motif of the Nur recognition box and a Nur/DNA complex model reveals that Nur mainly interacts with terminal bases of the palindrome on complex formation. This contrasts with more distributed contacts between Fur and the n-1-n type of the Fur-binding motif. The disparity between Nur and Fur in the conformation of the S1-S2 sheet in the DNA-binding domain can explain their different DNA-recognition patterns. Furthermore, the fact that the specificity of Nur in metal sensing and DNA recognition is conferred by the specific metal site suggests that its introduction drives the evolution of Nur orthologs in the Fur family.

Correspondence may also be addressed to Jung-Hye Roe. Tel: +82 2 880 6706; Fax: +82 2 888 4911; Email: jhroe{at}snu.ac.kr

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.


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