Skip Navigation



Nucleic Acids Research Advance Access published online on April 24, 2009
Nucleic Acids Research, doi:10.1093/nar/gkp253
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1966K) Freely available
Right arrow Screen PDF (1982K) Freely available
Right arrowOA All Versions of this Article:
37/suppl_2/W441    most recent
gkp253v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Cer, R. Z.
Right arrow Articles by Lebeda, F. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cer, R. Z.
Right arrow Articles by Lebeda, F. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published by Oxford University Press 2009
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Web Server Issue

IC50-to-Ki: a web-based tool for converting IC50 to Ki values for inhibitors of enzyme activity and ligand binding

R. Z. Cer1, U. Mudunuri1, R. Stephens1 and F. J. Lebeda2,*

1Advanced Biomedical Computing Center, Advanced Technology Program, SAIC-Frederick Inc., NCI-Frederick, Frederick, MD 21702, USA and 2US Army Medical Research Institute for Infectious Diseases, Fort Detrick, MD 21702-5011, USA

*To whom correspondence should be addressed. Tel: +1 301 619 4279; Fax: +1 301 619 2348; Email: frank.lebeda{at}amedd.army.mil

Received February 6, 2009. Revised March 25, 2009. Accepted April 5, 2009.

A new web-server tool estimates Ki values from experimentally determined IC50 values for inhibitors of enzymes and of binding reactions between macromolecules (e.g. proteins, polynucleic acids) and ligands. This converter was developed to enable end users to help gauge the quality of the underlying assumptions used in these calculations which depend on the type of mechanism of inhibitor action and the concentrations of the interacting molecular species. Additional calculations are performed for nonclassical, tightly bound inhibitors of enzyme-substrate or of macromolecule-ligand systems in which free, rather than total concentrations of the reacting species are required. Required user-defined input values include the total enzyme (or another target molecule) and substrate (or ligand) concentrations, the Km of the enzyme-substrate (or the Kd of the target-ligand) reaction, and the IC50 value. Assumptions and caveats for these calculations are discussed along with examples taken from the literature. The host database for this converter contains kinetic constants and other data for inhibitors of the proteolytic clostridial neurotoxins (http://botdb.abcc.ncifcrf.gov/toxin/kiConverter.jsp).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
L. M. Brinkac, T. Davidsen, E. Beck, A. Ganapathy, E. Caler, R. J. Dodson, A. S. Durkin, D. M. Harkins, H. Lorenzi, R. Madupu, et al.
Pathema: a clade-specific bioinformatics resource center for pathogen research
Nucleic Acids Res., October 20, 2009; (2009) gkp850v1.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.