Cover. In vivo selection of trans-splicing ribozymes optimized for target interaction in Saccharomyces cerevisiae, as described by Ayre et al. in this issue [Nucleic Acids Res. (2002) 30, e141]. A trans-splicing ribozyme library was created with randomized sequences at the 5' end, and sequences encoding a GAL4-VP16 trans-activator at the 3' end (top left). Optimal ribozymes interact with the intended target, and trans-splicing creates a chimeric transcript (middle left). GAL4-VP16 translated from this transcript promotes gene expression from GAL4-recognized upstream activating sequences (UAS), and enables growth on selective media and {beta}-galactosidase assays (bottom left and right).
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