Cover.
Unidirectional translocation on DNA by EcoP15I restriction enzyme. Inversely oriented recognition sites are shown as 5'-CAGCAG-3' and 5'-CTGCTG-3' on the same strand of DNA. The tetrameric holoenzyme is represented by two Mod subunits (in red) and two Res subunits (in yellow). The enzyme molecules bind to the sites and translocate on DNA in 5' to 3' direction of the site, utilizing ATP in the process. Two molecules interact on DNA to form a single endonucleolytic complex. The complex translocates towards one of the sites and cleaves DNA 25-27 bp downstream of the site. For further details see the paper by Raghavendra and Rao in this issue [Nucleic Acids Res. (2004) 32, 5703-5711].
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