Certain Cs within angiosperm chloroplast genome-encoded transcripts are edited to U. Tobacco plants containing chloroplast genomes transformed with a gene expressing a 92 nt region surrounding a C target of editing derived from chloroplast gene rpoB exhibit a normal wild-type appearance (upper right). Plants undergo abnormal development (lower left) and cannot survive outside of tissue culture when transformed with a 92 nt region followed by a 27 nt region perfectly complementary to sequences surrounding the C target of editing. In these plants, though the endogenous wild-type rpoB gene is edited normally in leaf tissue, the transgene transcripts are not edited at all. The hypothetical transgene transcript structure near the edited C is shown at lower right (red). For further details see the paper by Hegeman et al. in this issue: Nucleic Acids Research (2005), 33, 1454-1464.
[Table of Contents]