Cover: Screen for sequence-specific RNA-protein interaction in mammalian cells based on a bicistronic translational repression assay. A series of panels from upper left to lower right show the increasing ability of a sequence-specific RNA-binding protein within the context of a GFP fusion to repress cap-dependent translation of a red fluorescent protein, leading to absence of red fluorescence in the full panel as the wild-type level of RNA-binding and translational repression are achieved in the fusion protein. Presence of cyan fluorescence marks cells successfully transfected with a bicistronic reporter gene; whereas, presence of green fluorescence indicates successful production of an RNA-binding protein fused to GFP. Different amounts of red, green, and cyan fluorescence yield cells with a variety of colors (second panel on the lower right). Cyan fluorescence is pseudo-colored blue. (see the paper by Nie and Htun (2006), Nucleic Acids Res., 34, 5528–5540).
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