Cover: Central nucleotides within the 5'-CCNGG target sequence are flipped out in the restriction enzyme Ecl18kI-DNA co-crystal. 2-Aminopurine fluorescence studies demonstrate that C-EcoRII and PspGI restriction enzymes specific for the 5'-CCWGG sequence also flip central nucleotides to achieve specificity and adjust the cleavage pattern. For further details see the article by Tamulaitis et al. (Nucleic Acids Res., 35, 4792–4799). Cover image was generated by Giedre Tamulaitiene.
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